Biology/Higher Human S5 PCR
Description
This is a 1 day practical course based in our labs at FVC. Students will be trained in proper lab techniques below but also including the operation of P10, P200 and P1000 variable volume pipettes (Gilson). The theory of each of these practical will be explained on the day and students will be required to record scientific methods, conclusions, discussions, errors and evaluations.
Part 1 - Students will extract their own mitochondrial DNA and amplify it using PCR.
Students will be trained in the proper use of variable volume pipettes. Participating students will extract their own cheek cell mitochondrial DNA. Their DNA will be added to a master mix and the will amplify it up using our thermal cycler while reinforcing the theory behind this assay.
Gel electrophoresis of PCR products
After successful completion of the PCR, students will run samples on agarose gel and visualise under a UV light. Students will measure their bands using a DNA ladder and deduce the genetic similarities of mitochondrial DNA.
PPE
Lab coats and safety glasses will be available.
Prerequisites
Prior to students attending the short course, it would be helpful (but not essential) for them to receive some learning activities on PCR and gel electrophoresis
To save time, it would also be beneficial for students to fill in the electronic enrolment form.